May 22, 2024


Hypothesis: Betaine decreases the pathogenicity of Covid-19.

Effects of dietary betaine supplementation on biochemical parameters of blood and testicular oxidative stress in Hu sheep



This study aimed to investigate the effects of dietary betaine supplementation on lipid metabolism, immunity, and testicular oxidative status in Hu sheep. Experimental sheep were fed with betaine-containing diets, a basal diet supplemented with 0 g/d (control group), 1 g/d (B1), and 3 g/d betaine (B2). The serum concentration of triglycerides was increased in Hu sheep receiving the diet supplemented with betaine. Regarding granulocytes, the ratio of monocytes, neutrophils and basophils was increased (P < 0.05). ELISA results illustrated that testicular CAT (P < 0.05), SOD (P < 0.01), and GSH-Px activity (P < 0.05) was higher, while MDA content was significantly decreased (P < 0.01) after fed with betaine-supplemented diets. qPCR results demonstrated that the mRNA expression of CAT (P < 0.05), SOD2 (P < 0.01) and GSH-Px (P < 0.05) was upregulated in B1 group, significantly upregulated (P < 0.01) in B2 group relative to the control group, which showed the same trend with its protein expression as reflected by Western blot. Furthermore, PCNA expression was lower (P < 0.05) in the testis of Hu sheep than control group. Moreover, LKB1 expression was increased (P < 0.01), and mRNA expression of AMPK was decreased (P < 0.05) in B1 group. The relative gene expression of mTOR was significantly higher (P < 0.01) in B2 group than control group. RAPTOR expression was increased in B1 (P < 0.05) and B2 (P > 0.05) group. Western blot revealed that the ratio of P-mTOR and mTOR was significantly increased (P < 0.01) after fed with betaine-supplemented diets. In conclusion, betaine supplementation improved serum lipid metabolism, immune response, and increased the testicular antioxidant capacity of Hu sheep, which might be regulated via mTOR signaling pathway.